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Bulking generated considerable bias in detection of amplified fragment length polymorphism variations in oat,fringed brome and smooth bromegrass
Authors:Y B Fu  S Whitwill  Y S N Ferdinandez  B E Coulman  K W Richards
Abstract:Amplified fragment length polymorphism (AFLP) variations in bulk and plant‐by‐plant (PBP) samples of five oat (Avena sativa L.) accessions, one fringed brome (Bromus ciliatus L.) accession and two smooth bromegrass (B. inermis Leyss.) accessions were compared. The proportions of AFLP bands detected in PBP, but lost in bulk, samples of oat, fringed brome, and smooth bromegrass ranged from 19 to 31%, 40 to 44%, and 22 to 33% of the total bands scored, respectively. These lost bands had occurred at frequencies ranging from 0.1 to 1 in the PBP samples. These findings demonstrate bulking can generate substantial bias in detection of AFLP variations.
Keywords:AFLP  Avena  Bromus  bulking  genetic variation  oat
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