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A quantitative evaluation of two methods for preserving hair samples
Authors:D. A. Roon  L. P. Waits  K. C. Kendall
Abstract:Hair samples are an increasingly important DNA source for wildlife studies, yet optimal storage methods and DNA degradation rates have not been rigorously evaluated. We tested amplification success rates over a one‐year storage period for DNA extracted from brown bear (Ursus arctos) hair samples preserved using silica desiccation and ?20 °C freezing. For three nuclear DNA microsatellites, success rates decreased significantly after a six‐month time point, regardless of storage method. For a 1000 bp mitochondrial fragment, a similar decrease occurred after a two‐week time point. Minimizing delays between collection and DNA extraction will maximize success rates for hair‐based noninvasive genetic sampling projects.
Keywords:DNA extraction  DNA preservation  hair  noninvasive genetic sampling  PCR  Ursus arctos
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