DNA variants with telomere probe enable genetic mapping of ends of mouse chromosomes |
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Authors: | Rosemary W Elliott Chao-Huang Yen |
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Institution: | (1) Department of Molecular and Cell Biology, Roswell Park Cancer Institute, 14263 Buffalo, New York, USA |
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Abstract: | Dde I-digested DNA fragments from 11 inbred mouse strains were separated by electrophoresis, blotted and probed with a labeled
oligomer, TELO, containing five repeats of the consensus mammalian telomere sequence, TTAGGG. Each strain produced a unique
set of hybridizing fragments. Segregation analysis of TELO-hybridizing fragments from the BXD RI strains indicated that each
fragment segregated as expected for a single gene. One fragment from strain DBA/2J was genetically linked to locusXmv-9, previously mapped near the distal end of the map of chromosome (Chr) 4 and three fragments toCck, near the distal end of Chr 9, suggesting that these fragments are telomeric and represent the ends of the chromosome maps.
Confirmation of these map positions was obtained from a backcross. Fragments associated with the short arm of the Y Chr were
found in DNA from strains C57BL/6J and DBA/2J. TELO-hybridizing fragments from DBA/2J were digested by the exonuclease Bal
31, under conditions in which fragments hybridizing to a cDNA probe for themetallothioneine locus, located at the middle of mouse Chr 8, remained intact. Thus both biochemical and genetic tests indicate that several
TELO-hybridizing fragments fromDde I-digested DNA are at the ends of chromosomes and probably derive from mouse telomeres. Using this approach should allow
the mapping of genes relative to the ends of other mouse chromosomes. |
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