GAS CHROMATOGRAPHY-MASS FRAGMENTOGRAPHIC DETERMINATION OF INDOLE-3-ACETIC ACID IN RAT BRAIN |
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| Authors: | J J Warsh P W Chan D D Godse D V Coscina H C Stancer |
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| Institution: | Section on Neurochernistry, Clarke Institute of Psychiatry, University of Toronto, 250 College Street, Toronto, Ontario, M5T 1RS, Canada |
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| Abstract: | Abstract— A gas chromatography-mass fragmentographic procedure is described for the quantitation of indole-3-acetic acid (IAA) in rat brain. IAA was determined as its IV-1-pentafluoropropionyl-methyl ester. Deuterium labeled IAA was employed as an internal standard. Assay sensitivities of at least 500 pg and 2.5 ng could be achieved by single and double ion monitoring respectively. Whole brain IAA concentrations were determined to be 11.6 + 0.75 ng/g (n = 6). IAA concentrations in brain areas ranged from 10 to 64 ng/g with highest levels in the striatum. Rat brain IAA accumulated curvilinearly with respect to time over a 90 min interval following probenecid (200 mg/kg, i.p.). The efflux of IAA from brain appears mediated in part through a probenecid-sensitive active efflex process. |
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