Ryanodine receptor point mutations confer diamide insecticide resistance in tomato leafminer,Tuta absoluta (Lepidoptera: Gelechiidae) |
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| Institution: | 1. Hellenic Agricultural Organisation - ''Demeter'', Institute of Olive Tree, Subtropical Crops and Viticulture, Heraklion, Crete, Greece;2. Bayer CropScience, R&D Pest Control, Monheim, Germany;3. Department of Biology, Martin-Luther-Universität Halle-Wittenberg, Halle, Germany;4. Departamento de Producción Vegetal, Universidad Politécnica de Cartagena, Cartagena, Spain;5. Institute of Molecular Biology & Biotechnology, Foundation for Research & Technology Hellas, Crete, Greece;6. Departamento de Agronomia, Universidade Federal Rural de Pernambuco – UFRPE, Recife, Brazil;7. Rothamsted Research, Harpenden, Herts, UK;8. College of Life and Environmental Sciences, University of Exeter, Penryn, UK;9. Department of Crop Science, Agricultural University of Athens, Athens, Greece;1. Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, China;2. Department of Entomology, University of Kentucky, Lexington, KY, USA;1. Division of Molecular Entomology, ICAR-National Bureau of Agricultural Insect Resources, Bengaluru 560 024, Karnataka, India;2. Centre for Agricultural Bioinformatics, Indian Agricultural Statistical Research Institute, Pusa, New Delhi 110 012, India;1. Departamento de Agronomia – Entomologia, Universidade Federal Rural de Pernambuco, Av. Dom Manoel de Medeiros s/n, Dois Irmãos, 52171-900, Recife, PE Brazil;2. Departamento de Morfologia e Fisiologia Animal, Universidade Federal Rural de Pernambuco, Av. Dom Manoel de Medeiros s/n, Dois Irmãos, 52171-900, Recife, PE Brazil |
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| Abstract: | Insect ryanodine receptors (RyR) are the molecular target-site for the recently introduced diamide insecticides. Diamides are particularly active on Lepidoptera pests, including tomato leafminer, Tuta absoluta (Lepidoptera: Gelechiidae). High levels of diamide resistance were recently described in some European populations of T. absoluta, however, the mechanisms of resistance remained unknown. In this study the molecular basis of diamide resistance was investigated in a diamide resistant strain from Italy (IT-GELA-SD4), and additional resistant field populations collected in Greece, Spain and Brazil. The genetics of resistance was investigated by reciprocally crossing strain IT-GELA-SD4 with a susceptible strain and revealed an autosomal incompletely recessive mode of inheritance. To investigate the possible role of target-site mutations as known from diamondback moth (Plutella xylostella), we sequenced respective domains of the RyR gene of T. absoluta. Genotyping of individuals of IT-GELA-SD4 and field-collected strains showing different levels of diamide resistance revealed the presence of G4903E and I4746M RyR target-site mutations. These amino acid substitutions correspond to those recently described for diamide resistant diamondback moth, i.e. G4946E and I4790M. We also detected two novel mutations, G4903V and I4746T, in some of the resistant T. absoluta strains. Radioligand binding studies with thoracic membrane preparations of the IT-GELA-SD4 strain provided functional evidence that these mutations alter the affinity of the RyR to diamides. In combination with previous work on P. xylostella our study highlights the importance of position G4903 (G4946 in P. xylostella) of the insect RyR in defining sensitivity to diamides. The discovery of diamide resistance mutations in T. absoluta populations of diverse geographic origin has serious implications for the efficacy of diamides under applied conditions. The implementation of appropriate resistance management strategies is strongly advised to delay the further spread of resistance. |
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| Keywords: | Diamide resistance Flubendiamide Chlorantraniliprole Ryanodine receptor Target-site mutation |
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