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The global dynamic of DNA methylation in response to heat stress revealed epigenetic mechanism of heat acclimation in Saccharina japonica |
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| Authors: | Fuli Liu Pengyan Zhang Zhourui Liang Yanmin Yuan Yi Liu Yukun Wu |
| Affiliation: | 1. Key Laboratory of Marine Genetics and Breeding, Ministry of Education;2. College of Marine Life Science, Ocean University of China, Qingdao, China;3. Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture;4. Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China Contribution: Data curation (equal), Methodology (equal);5. Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China Contribution: Conceptualization (equal), Methodology (equal), Validation (equal);6. Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China Contribution: Data curation (equal), Formal analysis (equal), Investigation (equal);7. Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China Contribution: Data curation (equal), Methodology (equal), Resources (equal) |
| Abstract: | Saccharina japonica is an ecologically and economically important kelp in cold-temperate regions. When it is cultivated on a large scale in the temperate and even subtropical zones, heat stress is a frequent abiotic stress. This study is the first attempt to reveal the regulatory mechanism of the response to heat stress from the perspective of DNA methylation in S. japonica. We firstly obtained the characteristics of variation in the methylome under heat stress, and observed that heat stress caused a slight increase in the overall methylation level and methylation rate, especially in the non-coding regions of the genome. Secondly, we noted that methylation was probably one of factors affecting the expression of genes, and that methylation within the gene body was positively correlated with the gene expression (rho = 0.0784). Moreover, it was found that among the differentially expressed genes regulated by methylation, many genes were related to heat stress response, such as HSP gene family, genes of antioxidant enzymes, genes related to proteasome-ubiquitination pathway, and plant cell signaling pathways. This study demonstrated that DNA methylation is involved in regulating the response to heat stress, laying a foundation for studying the acclimation and adaptation of S. japonica to heat stress from an epigenetic perspective. |
| Keywords: | differentially expressed gene DNA methylation heat stress RNA-seq Saccharina japonica whole-genome bisulfite sequencing |