An isoform of Taiman that contains a PRD-repeat motif is indispensable for transducing the vitellogenic juvenile hormone signal in Locusta migratoria |
| |
| Affiliation: | 1. State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China;2. State Key Laboratory of Cotton Biology, Institute of Plant Stress Biology, School of Life Sciences, Henan University, Kaifeng 475004, China;3. University of Chinese Academy of Sciences, Beijing 100049, China;1. School of Economics, Henan University, China;2. Halle Institute for Economic Research, Germany;1. University of Minnesota at Morris, Morris, MN 56267, USA;2. School of Economics, Henan University, Ming Lun Street, Kaifeng, Henan 475001, China;1. State Key Laboratory for Oxo Synthesis and Selective Oxidation, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, PR China;2. Graduate University of Chinese Academy of Sciences, Beijing 100049, PR China;1. Department of Agricultural Economics, Texas A&M University, 2124 TAMU, College Station, TX 77843-2124, USA;2. School of Economics, Henan University, Kaifeng, Henan 475000, China;1. College of Economics, Henan University, China;2. Faculty of Business Administration, University of Macau, China;3. CRPE and College of Economics, Zhejiang University, China;1. Henan University, School of Economics, Kaifeng, China;2. Halle Institute for Economic Research (IWH), Department of Macroeconomics, Halle, Germany;3. Martin-Luther University Halle-Wittenberg, Chair of Macroeconomics, Halle, Germany |
| |
| Abstract: | Taiman (Tai) has been recently identified as the dimerizing partner of juvenile hormone (JH) receptor, Methoprene-tolerant (Met). However, the role of Tai isoforms in transducing vitellogenic signal of JH has not been determined. In this study, we show that the migratory locust Locusta migratoria has two Tai isoforms, which differ in an INDEL-1 domain with the PRD-repeat motif rich in histidine and proline at the C-terminus. Tai-A with the INDEL-1 is expressed at levels about 50-fold higher than Tai-B without the INDEL-1 in the fat body of vitellogenic adult females. Knockdown of Tai-A but not Tai-B results in a substantial reduction of vitellogenin expression in the fat body accompanied by the arrest of ovarian development and oocyte maturation, similar to that caused by depletion of both Tai isoforms. Either Tai-A or Tai-B combined with Met can induce target gene transcription in response to JH, but Tai-A appears to mediate a significantly higher transactivation. Our data suggest that the INDEL-1 domain plays a critical role in Tai function during reproduction as Tai-A appears be more active than Tai-B in transducing the vitellogenic JH signal in L. migratoria. |
| |
| Keywords: | Taiman isoforms INDEL-1/PRD motif Juvenile hormone Vitellogenesis Oocyte maturation |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
