Dissecting of the FHB resistance QTL on the short arm of wheat chromosome 2D using a comparative genomic approach: from QTL to candidate gene |
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Authors: | Hirokazu Handa Nobukazu Namiki Donghe Xu Tomohiro Ban |
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Institution: | (1) Plant Genome Research Unit, National Institute of Agrobiological Sciences, 2-1-2, Kan-non-dai, Tsukuba 305-8602, Japan;(2) Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8577, Japan;(3) Institute of the Society for Techno-innovation of Agriculture, Forestry and Fisheries (STAFF), Tsukuba 305-0854, Japan;(4) Japan International Agricultural Research Center for Agricultural Sciences (JIRCAS), Tsukuba 305-8686, Japan;(5) Japan-CIMMYT FHB Project, CIMMYT (International Maize and Wheat Improvement Center), Mexico, DF, 06600, Mexico;(6) Kihara Institute of Biological Research, Yokohama City University, Yokohama 244-0813, Japan |
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Abstract: | Colinearity in gene content and order between rice and closely related cereal crops has been a powerful tool for gene identification.
Using a comparative genomic approach, we have identified the rice genomic region syntenous to the region of the short arm
of wheat chromosome 2D, on which quantitative trait loci (QTLs) for Fusarium head blight (FHB) resistance and for controlling accumulation of the mycotoxin deoxynivalenol (DON) are closely located.
Utilizing markers known to reside near the FHB resistance QTL and data from several wheat genetic maps, we have limited the
syntenous region to 6.8 Mb of the short arm of rice chromosome 4. From the 6.8-Mb sequence of rice chromosome 4, we found
three putative rice genes that could have a role in detoxification of mycotoxins. DNA sequences of these putative rice genes
were used in BLAST searches to identify wheat expressed sequence tags (ESTs) exhibiting significant similarity. Combined data
from expression analysis and gene mapping of wheat homologues and results of analysis of DON accumulation using doubled haploid
populations revealed that a putative gene for multidrug resistance-associated protein (MRP) is a possible candidate for the
FHB resistance and/or DON accumulation controlling QTLs on wheat chromosome 2DS and can be used as a molecular marker to eliminate
the susceptible allele when the Chinese wheat variety Sumai 3 is used as a resistance source.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. |
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Keywords: | Comparative genomics Fusarium head blight (FHB) Multidrug resistance-associated protein (MRP) Mycotoxin QTL Wheat |
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