The use of an immunological probe to measure the kinetics of DNA repair in normal and UV-sensitive mammalian cell lines |
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| Affiliation: | 1. Department of Chemical and Biomolecular Engineering, Yonsei University, Seoul 03722, Republic of Korea;2. Green Materials and Processes R&D Group, Korea Institute of Industrial Technology, Ulsan 44413, Republic of Korea;3. Department of Chemical and Biomolecular Engineering, Seoul National University of Science and Technology, Seoul 01811, Republic of Korea;1. Institute of Rare Diseases, West China Hospital of Sichuan University, Chengdu, Sichuan 610041, China;2. Key Laboratory of Systems Health Science of Zhejiang Province, School of Life Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou, Zhejiang 310024, China;3. Key Laboratory of RNA Biology, Center for Big Data Research in Health, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China;4. University of Chinese Academy of Sciences, Beijing 100049, China;5. State Key Laboratory of Molecular Biology, State Key Laboratory of Cell Biology, Shanghai Key Laboratory of Molecular Andrology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, Shanghai 200031, China;1. Department of Biotechnology, Yonsei University, Seoul 03722, Republic of Korea;2. Center for Nanomedicine, Institute for Basic Science (IBS), Seoul 03722, Republic of Korea;3. Graduate Program of Nano Biomedical Engineering (NanoBME), Advanced Science Institute, Yonsei University, Seoul 03722, Republic of Korea;1. Department of Naval Architecture and Ocean Engineering, Seoul National University, Seoul, Republic of Korea;2. Institute of Engineering Research & Research Institute of Marine Systems Engineering, Seoul National University, Seoul, Republic of Korea;3. Ocean and Ship Research Group, Department of Civil and Environmental Engineering, Petronas University of Technology, Seri Iskandar, Perak, Malaysia;4. Department of Maritime Technology, Universiti Malaysia Terengganu, Kuala Nerus, Malaysia;5. Department of Manufacturing Systems and Design Engineering, Seoul National University of Science and Technology (SeoulTech), Seoul, Republic of Korea;1. Department of Civil and Environmental Engineering, Konkuk University, Seoul 05029, Republic of Korea;2. School of Urban and Environmental Engineering, Ulsan National Institute of Science and Technology (UNIST), UNIST-gil 50, Ulsan 44919, Republic of Korea;3. School of Civil, Environmental, and Architectural Engineering, Korea University, Seoul 02841, South Korea;4. Department of Integrated Energy and Infra system, Kangwon National University, Kangwondaehak-gil, 1, Chuncheon-si, Gangwon-do 24341, Republic of Korea;5. Department of Environmental Engineering, College of Engineering, Kangwon National University, Kangwondaehak-gil, 1, Chuncheon-si, Gangwon-do 24341, Republic of Korea;1. Division of Genetics, Department of Medicine, Brigham & Women''s Hospital, Boston, Massachusetts, USA;2. Harvard Medical School, Boston, Massachusetts, USA;3. Transplant Research Program, Division of Nephrology, Boston Children''s Hospital, Boston, Massachusetts, USA;4. Broad Institute, Cambridge, Massachusetts, USA;5. Division of Pediatric Blood and Marrow Transplantation & Cellular Therapy, Department of Pediatrics, University of Minnesota Twin Cities, Minneapolis, Minnesota, USA;6. Harvard Stem Cell Institute, Harvard University, Cambridge, Massachusetts, USA;7. Department of Dermatology, Brigham & Women''s Hospital, Boston, Massachusetts, USA;8. Medical School, University of Minnesota Twin Cities, Minneapolis, Minnesota, USA;9. Stem Cell Institute, Medical School, University of Minnesota Twin Cities, Minneapolis, Minnesota, USA;10. Department of Dermatology, Medical School, University of Minnesota Twin Cities, Minneapolis, Minnesota, USA;11. Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, Massachusetts, USA;12. Department of Medicine, King Saud bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia;13. Department of Medicine, VA Boston Healthcare System, West Roxbury, Massachusetts, USA |
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| Abstract: | Chinese hamster ovary cells and human fibroblasts were used to study UV-light-induced repair replication and removal of antibody-binding sites. Whereas repair replication still continued 8 h post irradiation, removal of antibody-binding sites was 80% complete within 2 h and reached a plateau by 4 h. This was found to be independent of the method of DNA isolation. UV-hypersensitive CHO cells exhibited reduced levels of repair synthesis that closely correlated with the extent of removal of antibody-binding sites. XP group A, C and D cells, each of which had less than 15% of the level of repair synthesis found in the control fibroblasts, removed less than 30% of the antibody-binding sites. Group E cells demonstrated intermediate levels of DNA-repair capacity in both assays. |
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