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Hydrolysis of glycol chitin by chitinolytic enzymes
Affiliation:2. US Grain Marketing Research Laboratory, Agricultural Research Service, US Department of Agriculture, Manhattan, KS 66502, USA (Tel: 913 539-9141);3. Department of Agricultural Chemistry, Yamaguchi University, Yamaguchi 753 Japan;4. Department of Biochemistry, Kansas State University, Manhattan, KS 66506, USA;1. Mechanical Engineering Department, University of Hormozgan, Bandar Abbas, Iran;2. Khuzestan Province Gas Company, Ahvaz, Iran;1. Ferrous Alloy Department, Advanced Metallic Materials Division, Korea Institute of Materials Science, 797 Changwondae-ro, Seongsan-gu, Changwon, Gyeongnam, 51508, Republic of Korea;2. Department of Materials Science and Engineering and RIAM, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 08826, Republic of Korea;3. Department of Materials Science and Engineering, Changwon National University, 20 Changwondaehak-ro, Uichang-gu, Changwon, Gyeongnam, 51140, Republic of Korea;1. State Key Laboratory of Solidification Processing, Northwestern Polytechnical University, Xi’an 710072, China;2. National & Local Joint Engineering Research Center for Precision Thermoforming Technology of Advanced Metal Materials, Xi’an, Shaanxi 710072, China;3. AVIC Manufacturing Technology Institute, Beijing 100024, China;1. Department of Culture Studies, Tilburg University, the Netherlands;2. Department of Psychology, İzmir Kâtip Çelebi Üniversitesi, Turkey
Abstract:
  • 1.1. The hydrolysis of glycol chitin preparations by several β-N-acetylglucosaminidases was monitored colorimetrically with the potassium ferriferrocyanide reagent.
  • 2.2. Glycol chitin samples from crab and insect sources varied considerably in chemical composition and susceptibility to enzymatic hydrolysis.
  • 3.3. Insect endochitinase preferred crab glycol chitin as substrate while hen's egg white lysozyme preferred commercial glycol chitin.
  • 4.4. Insect glycol chitin was well hydrolyzed by both enzymes.
  • 5.5. Insect exochitinase did not digest glycol chitin.
Keywords:
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