Water exchange through erythrocyte membranes V. Incubation with papain prevents the p-chloromercuri-benzensulfonate inhibition of water diffusion studied by a nuclear magnetic resonance technique |
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| Affiliation: | 1. Department of Biochemistry, Purdue University, West Lafayette, IN 47907, USA;2. Purdue University Center for Cancer Research, West Lafayette, IN 47907, USA;1. Laboratory of Climate-Smart Food Crop Production, Institute of Tropical Agriculture and Food Security, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia;2. Laboratory of Plantation Science and Technology, Institute of Plantation Studies, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia;3. Department of Land Management, Faculty of Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia;4. Mayo Clinic, Rochester, Minnesota, USA;5. Department of Molecular Biology & Biotecnology, University of Sheffield, United Kingdom;6. Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia;7. Malaysia Genome Institute, Jalan Bangi, 43000 Kajang, Malaysia |
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| Abstract: | The changes in water diffusion across human erythrocyte membrane following exposure to proteolytic enzymes and to p-chloromercuribenzene sulfonate (PCMBS) have been studied on isolated erythrocytes suspended in isotonic solutions. Trypsin digested glycophorin without significantly changing the pattern of other polypeptides in erythrocyte membrane. On the contrary, with chymotrypsin or papain an extensive digestion of band 3 protein occured. No changes in water diffusion were noticed after exposure of erythrocytes to trypsin, chymotrypsin or papain. Neither trypsin nor chymotrypsin treatment prevented the inhibition of water diffusion induced by PCMBS. In contrast, exposure of erythrocytes to papain did hamper the inhibitory effect of subsequent incubation with PCMBS. Taking into account the degradation of band 3 protein by papain it appears that the binding site for PCMBS playing a role in the inhibition of water diffusion is located in this protein. |
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