The extracellular proteolytic enzymes of the mosquito-parasitizing fungus Lagenidium giganteum |
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| Affiliation: | 1. Department of Traditional Chinese and Western Medicine, Xianyang Caihong Hospital, Xianyang City, Shanxi Province 712021, PR China;2. Department of Clinical Laboratory, Chinese People''s Liberation Army Third Hospital, Baoji City, Shanxi Province 721004, PR China;3. Department of Clinical Laboratory, The First Affiliated Hospital of Xi ''an Medical College, Xian City, Shanxi Province 710021, PR China;4. Department of Clinical Laboratory, Shaanxi People''s Hospital, Xian City, Shanxi Province 710068, PR China |
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| Abstract: | Lagenidium giganteum produces extracellular proteases when grown in peptone-yeast extract-glucose medium. The production of the enzymes required a suitable inducer such as protein, and was repressed by glucose. The total filtrate proteases had a pH optimum of 8.4, and were heat denatured t 60°C. The use of specific substrates and inhibitors demonstrated the presence of collagenase, trypsin-like protease, and a weak elastase. Trypsin-like activity was present in the medium after 38 h of growth, whereas collagenase appeared in 43 h. The roles of these enzymes in pathogenesis were discussed. |
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