X-ray-sensitive mutants of Chinese hamster ovary cell line isolation and cross-sensitivity to other DNA-damaging agents |
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| Affiliation: | 1. Department of Pathology, Yale University School of Medicine, New Haven, CT, USA;1. Pharmaceutical Biotechnology Research Group, Faculty of Pharmacy, Universitas Indonesia, Kampus UI Depok, Depok 16424, Indonesia;2. Graduate School of Biological Sciences, Nara Institute of Science and Technology (NAIST), 8916-5 Takayama, Ikoma, Nara 630-0101, Japan;1. University of Girona, Campus Montilivi, Edif. PII, E17003, Girona, Catalonia, Spain;2. Institut de Ciència de Materials de Barcelona, ICMAB – CSIC, Campus UA Barcelona, E-08193, Bellaterra, Catalonia, Spain;1. Department of Chemistry, National Institute of Technology, Agartala 799046, Tripura, India;2. Departamento de Química, Universitat de les llles Balears, Crta. de Valldemossa, 07122, Spain;3. Centro de Química Estrutural, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-1005 Lisbon, Portugal;4. Division of Chemistry & Biological Chemistry, Nanyang Technology University, Singapore 639798, Singapore;5. Faculty of Chemistry, University of Wroclaw, Wroclaw 50383, Poland |
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| Abstract: | A standard technique of microbial genetics, which involves the transfer of cells from single colonies by means of sterile toothpicks, has been adapted to somatic cell genetics. Its use has been demonstrated in the isolation of X-ray-sensitive mutants of CHO cells. 9000 colonies have been tested and 6 appreciably X-ray-sensitive mutants were isolated. (D10 values 5–10-fold of wild-type D10 value.) A further 6 mutants were obtained which showed a slight level of sensitivity (D10 values less than 2-fold of wild-type D10 value).The 6 more sensitive mutants were also sensitive to bleomycin, a chemotherapeutic agent inducing X-ray-like damage. Cross-sensitivity to UV-irradiation and treatment with the alkylating agents, MMS, EMS and MNNG, was investigated for these mutants. Some sensitivity to these other agents was observed, but in all cases it was less severe than the level of sensitivity to X-irradiation. Each mutant showed a different overall response to the spectrum of agents examined and these appear to represent new mutant phenotypes derived from cultured mammalian cell lines. One mutant strain, xrs-7, was cross-sensitive to all the DNA-damaging agents, but was proficient in the repair of single-strand breaks. |
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