Mutagenicity of mono-, di- and tri-nitropyrenes in Chinese hamster ovary cells |
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| Affiliation: | 1. Institute of Experimental Physiology (IFISE-CONICET), Suipacha 570, 2000 Rosario, Argentina;2. University of Connecticut, School of Pharmacy, Department of Pharmaceutical Sciences, Storrs, CT, USA;3. Institute of Pharmacological Investigations (ININFA-CONICET), University of Buenos Aires, Buenos Aires, Argentina;4. Department of Clinical Pharmacology and Pharmacoepidemiology, University of Heidelberg, Heidelberg, Germany;1. Department of Infectious Diseases and;2. Department of Gastroenterology and Hepatology, The First Hospital of Lanzhou University, The First Medical College of Lanzhou University, Lanzhou University, Lanzhou, Gansu, China;3. Department of General Surgery, Lanzhou University Second Hospital, The Second Medical College of Lanzhou University, Lanzhou University, Lanzhou, Gansu, China;1. Department of Neurosurgery, The Fourth Affiliated Hospital, Harbin Medical University, Harbin, China;2. Department of Immunology, School of Basic Medical Sciences, Fudan University, Shanghai, China;3. Department of Neurosurgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, China;4. Department of Clinical Laboratory, Beijing Shijiantan Hospital, Capital Medical University, Beijing, China;5. Department of General Surgery, Shanghai Chang Zheng Hospital, Second Military Medical University, Shanghai, China;1. Department of Radiology, Qilu Hospital of Shandong University, Jinan, China;2. Department of Radiotherapy, Qilu Hospital of Shandong University, Jinan, China |
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| Abstract: | 1-Nitropyrene (1-NP), 1,3-dinitropyrene (1,3-DNP), 1,6-dinitropyrene (1,6-DNP), 1,8-dinitropyrene (1,8-DNP) and 1,3,6-trinitropyrene (1,3,6-TNP) were tested for mutagenicity in cultured Chinese hamster ovary (CHO) cells. Mutation at the hypoxanthine-guanine phosphoribosyl transferase gene locus was quantified. While 1-NP and 1,3-DNP had only marginal direct-acting mutagenicity, 1,6-DNP, 1,8-DNP and 1,3,6-TNP showed definite mutagenicity, with specific mutagenic activities of 8.1, 21 and 54 mutants/106 survivors/μg·ml−1 respectively. The mutagenicity of 1-NP increased with increasing concentrations of Aroclor-1254 induced liver homogenate (S9) in the treatment medium. However, S9 at all concentrations tested decreased the mutagenicity of 1,6-DNP and 1,8-DNP. S9 at low concentrations enhanced the mutagenicity of 1,3-DNP and 1,3,6-TNP and that at high concentrations decreased their mutagenicity. The positive mutagenic response of the nitropyrenes suggests that they are potentially carcinogenic, and that further research into their possible human health risk should be performed. |
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