斑节对虾杆状病毒包涵体研究

利用显微图像分析技术,对斑节对虾肝胰腺正常细胞胞核和斑节对虾杆状病毒(MBV)感染细胞胞核横切面的直径、周长和面积进行了测量分析.结果表明:正常细胞胞核的直径为3.47±0.30μm、周长为13.03±1.36μm、面积为10.87±1.78μm²;MBV感染细胞胞核的直径为3.81±0.79μm、周长为14.00±2.87μm、面积为13.52±5.37μm²;两者的直径、周长和面积均存在极显著的差异(P<0.01).对3种类型的包涵体进行了测量,其中1类包涵体最大,2类包涵体次之,3类包涵体最小,并以1类包涵体占大多数.

Study on occlusion body of Penaeus monodon baculovirus

The diameters、perimeters and areas of normal cell nucleus and cell nucleus infected with Penaeus monodon baculovirus(MBV) in hepatopancreas epithelial cells of Penaeus monodon were studied by micro-imaging analytical technique.The results show that the mean diameter is 3.47±0.30 μm,the mean perimeter is 13.03±1.36 μm and the mean area is 10.87±1.78 μm² in normal cell nucleus,while the mean diameter is 3.81±0.79 μm,the mean perimeter is 14.00±2.87 μm and the mean area is 13.52±5.37 μm² in the cell nucleus infected with MBV.There was significant difference between them(P<0.01).Three type occlusion bodies(OB) were measured.The biggest OB is type I and the smallest OB is type III.The most common OB is type I.