Isolation and characterization of di- and tri-mannosyl-cyclomaltoheptaoses (beta-cyclodextrins) produced by reverse action of alpha-mannosidase from jack bean |
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| Authors: | Koizumi K Tanimoto T Okada Y Takeyama S Hamayasu K Hashimoto H Kitahata S |
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| Affiliation: | School of Pharmaceutical Sciences, Mukogawa Women's University, Nishinomiya, Japan. |
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| Abstract: | Di- and tri-mannosyl-cyclomaltoheptaoses (beta-cyclodextrins, beta CDs), which were synthesized together with monomannosyl-beta CD in a large-scale production by reverse action of alpha-mannosidase from jack bean, were isolated and purified by HPLC. The structures of seven isomers of di-mannosyl-beta CD and six isomers of tri-mannosyl-beta CD were elucidated by FABMS and NMR spectroscopy, and by enzymatic methods. |
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