北五味子及其不同炮制品中五味子甲素 和乙素的含量测定

目的:测定北五味子及其不同炮制品中五味子甲素、五味子乙素的含量。方法:采用高效液相色谱法,Spherisorb 10ODS1(4.6mm×250mm,10μm),以甲醇-水(70:30)为流动相,检测波长为254nm。线性范围0.1~0.5μg,相关系数为0.995.平均回收率为100.3%和100.4%,RSD=0.87%和2.85%(n=5)。结果:五味子甲素和乙素在生品、醋炙、炒制、酒炙、蒸炙、蜜炙品中的平均含量分别为0.13%,0.064%,0.052%,0.064%,0.11%,0.13%和0.21%,0.298%,0.238%,0.302%,0.216%,0.214%。结论:方法准确,快速,重现性好,可用于五味子各种炮制品的质量评价。

Determination of content of deoxyschizandrin and- schizandrin in the different processed productss of Fructus Schisandrae Chinensis by HPLC

Objective: To compare the content of deoxyschizandrin and??- schizandrin in the raw medical material and the different processd products of Fructus Schisandrae Chinensis( FSC) . Methods: The content of deoxyschizandrin and??- schizandrinwere was determined by HPLC( = high performance liquid chromatography ) , with Spherisorb 10 ODS1( 4. 6mm ?? 250mm, 10??m) , the mobile phase of a mixture of methanol- water( 70: 30) , and detecting wave length of 254nm. The standard curve was linear in the range of 0. 11~ 0. 55??g , with correlation coefficient 0. 995. The average recovery of FSC was 100. 3% and 100. 4% , with RSD= 0. 87% and 2. 85%. Results: The mean content of deoxyschizandrin was 0. 13%, 0. 064%, 0. 052%, 0. 064%, 0. 11%, 0. 13% and??- schizandrin, 0. 21%, 0. 298%, 0. 238% , 0. 302%, 0. 216%, 0. 214%, in the different processed products of FSC. Conclusion: The method is accurate, quick and good reappearance, which can be used for the quality evaluation in the different processed products of FSC.