Enhanced production of a thermostable mannanase by immobilized cells of <Emphasis Type="Italic">Bacillus</Emphasis><Emphasis Type="Italic">subtilis</Emphasis> on various membranes |
| |
| Authors: | Qiaojuan Yan Aimin Wu Zhengqiang Jiang Luo Tang Shi Bo |
| |
| Institution: | (1) Bioresource Utilization Laboratory, College of Engineering, China Agricultural University, 100083 Beijing, China;(2) Department of Biotechnology, College of Food Science and Nutritional Engineering, China Agricultural University, P.O. Box 294, No.17, Qinghua Donglu, 100083 Haidian District, Beijing, China;(3) Feed Research Institute, Chinese Academy of Agricultural Sciences, 100081 Beijing, China |
| |
| Abstract: | Cells of the thermophilic Bacillus subtilis WY34 were immobilized on various formaldehyde-activated polymer membranes and the immobilized cells were used for the production
of thermostable mannanase in flasks. The results showed that polyethersulfone membranes (PES) and nylon-6 membranes were the
most suitable supports for cell immobilization to produce the mannanase. Moreover, PES and nylon-6 membranes immobilized cells
provided 1.78- and 1.74-fold higher mannanase activity compared to the control after 4 days of cultivation, respectively.
The immobilized cells on PES and nylon-6 membranes had good stability and retained 131.5 and 114.3% of ability of enzyme production
even after six cycles of repeated batch fermentation, respectively. Active cell growth was observed by scanning electron microscopy
(SEM) after 16 days (four cycles) repeated batch cultivation. Therefore, the membrane-immobilized cells of B. subtilis WY34 can be proposed as an effective biocatalyst for repeated usage for production of the thermostable mannanase. |
| |
| Keywords: | Bacillus subtilis Enzyme production Immobilization Immobilized cells Mannanase Membrane |
| 本文献已被 SpringerLink 等数据库收录！ |
|
