Production of 7, 10-dihydroxy-8(<Emphasis Type="Italic">E</Emphasis>)-octadecenoic acid from triolein via lipase induction by <Emphasis Type="Italic">Pseudomonas aeruginosa</Emphasis> PR3 |
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Authors: | In-Ae Chang In-Hwan Kim Sun-Chul Kang Ching T Hou Hak-Ryul Kim |
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Institution: | (1) Department of Animal Science and Biotechnology, Kyungpook National University, 702-701 Daegu, South Korea;(2) Department of Food and Nutrition, College of Health Science, Korea University, Seoul, South Korea;(3) Department of Biotechnology, College of Engineering, Daegu University, Gyungsan, South Korea;(4) Microbial Genomic and Bioprocessing Research Unit, National Center for Agricultural Utilization Research, ARS, USDA, Peoria, IL 61604, USA |
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Abstract: | Hydroxy fatty acids (HFA) have gained importance because of their special properties such as higher viscosity and reactivity
compared with other non-hydroxy fatty acids. The bacterial isolate Pseudomonas aeruginosa (PR3) was reported to produce mono-, di-, and trihydroxy fatty acids from different unsaturated fatty acids. Of those, 7,10-dihydroxy-8(E)-octadecenoic acid (DOD) was produced with high yield from oleic acid by PR3. Up to now, the substrates used for microbial
HFA production were free fatty acids. However, it is possible to utilize triacylglycerides, specifically triolein containing
three oleic groups, as a substrate by microbial enzyme system involved in HFA production from oleic acid. In this study we
used triolein as a substrate and firstly report that triolein could be efficiently utilized by PR3 to produce DOD. Triolein
was first hydrolyzed into oleic acid by the triolein-induced lipase and then the released oleic acid was converted to DOD
by PR3. Results from this study demonstrated that natural vegetable oils, without being intentionally hydrolyzed, could be
used as efficient substrates for the microbial production of value-added hydroxy fatty acids. |
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Keywords: | Pseudomonas aeruginosa PR3 Hydroxy fatty acid Triolein Lipase Bioconversion Dihydroxy fatty acid |
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