|
|||||
|
|
Adiponectin AS lncRNA inhibits adipogenesis by transferring from nucleus to cytoplasm and attenuating Adiponectin mRNA translation |
|
| Authors: | Rui Cai Yunmei Sun Naren Qimuge Guoqiang Wang Yingqian Wang Guiyan Chu Taiyong Yu Gongshe Yang Weijun Pang |
| Affiliation: | Laboratory of Animal Fat Deposition & Muscle Development, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China |
| Abstract: | Adiponectin (AdipoQ) is an adipocyte-derived hormone with positive function on systemic glucose and lipid metabolism. Long noncoding RNA (lncRNA) is emerging as a vital regulator of adipogenesis. However, AdipoQ-related lncRNAs in lipid metabolism have not been explored. Here, AdipoQ antisense (AS) lncRNA was first identified, and we further found that it inhibited adipogenesis. The half-life of AdipoQ AS lncRNA was 10?h, whereas that of AdipoQ mRNA was 4?h. During adipogenic differentiation, AdipoQ AS lncRNA translocated from nucleus to cytoplasm. AdipoQ AS lncRNA and AdipoQ mRNA formed an RNA duplex. Moreover, AdipoQ AS lncRNA delivered via injection of adenovirus expressing AdipoQ AS lncRNA decreases white adipose tissue (WAT), brown adipose tissue (BAT) and liver triglycerides (TG) in mice consuming a high fat diet (HFD). Interestingly, the non-overlapping region of AdipoQ AS lncRNA improved serum glucose tolerance and insulin sensitivity in HFD mice, but not AdipoQ AS lncRNA. In conclusion, AdipoQ AS lncRNA transfer from nucleus to cytoplasm inhibits adipogenesis through formation of an AdipoQ AS lncRNA/AdipoQ mRNA duplex to suppress the translation of AdipoQ mRNA. Taken together, we suggest that AdipoQ AS lncRNA is a novel therapeutic target for obesity-related metabolic diseases. |
| Keywords: | AdipoQ Adiponectin AS lncRNA antisense long noncoding RNA FISH RPA ribonuclease protection assay iWAT inguinal white adipose tissue eWAT epididymal white adipose tissue BAT brown adipose tissue TG triglyceride HFD high fat diet RNA-Seq RNA sequencing FFAs free fatty acids GTT glucose tolerance test ITT insulin tolerance test CPC Coding Potential Calculator CPAT Coding Potential Assessment Tool NOR Ad-AS Ad-NOR adenovirus expressing NOR insulin receptor insulin receptor substrate peroxisome proliferator activated receptor γ CCAAT/enhancer binding protein α fatty acid binding protein 4 carnitine palmitoyl transferase 1 α hormone sensitive lipase adipose triglyceride lipase sterol responsible element binding protein 1c tumor necrosis factor α uncoupling protein 1 peroxisome proliferator activated receptor gamma coactivator-1 alpha PRD1-BF1-RIZ1 homologous domain-containing protein 16 FISH Half-life Adipogenesis RNA duplex Lipid metabolism |
| 本文献已被 ScienceDirect 等数据库收录！ | |