Effect of oregano essential oil and carvacrol on Cryptosporidium parvum infectivity in HCT-8 cells |
| |
Authors: | Shashank Gaur Theresa B. Kuhlenschmidt Mark S. Kuhlenschmidt Juan E. Andrade |
| |
Affiliation: | 1. Department of Food Science and Human Nutrition, the University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA;2. Department of Pathobiology, the University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA;3. Division of Nutritional Sciences, the University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA |
| |
Abstract: | Cryptosporidium parvum is the second leading cause of persistent diarrhea among children in low-resource settings. This study examined the effect of oregano essential oil (OEO) and carvacrol (CV) on inhibition of C. parvum infectivity in vitro. HCT-8 cells were seeded (1 × 106) in 96-well microtiter plates until confluency. Cell viability and infectivity were assessed by seeding HCT-8 cell monolayers with C. parvum oocysts (1 × 104) in two modalities: 1) 4 h co-culture with bioactive (0–250 μg/mL) followed by washing and incubation (48 h, 37 °C, 5% CO2) in bioactive-free media; and 2) 4 h co-culture of C. parvum oocysts followed by washing and treatment with bioactive (0–250 μg/mL) during 48-h incubation. Cell viability was tested using Live/Dead? assay whereas infectivity was measured using C. parvum-specific antibody staining via immunofluorescence detection. Loss of cell viability was observed starting at 125 μg/mL and 60 μg/mL for OEO and CV, respectively. Neither OEO nor CV modulated the invasion of C. parvum sporozoites in HCT-8 cells. Treatment with bioactive after invasion reduced relative C. parvum infectivity in a dose-dependent manner to 55.6 ± 10.4% and 45.8 ± 4.1% at 60 and 30 μg/mL of OEO and CV, respectively. OEO and CV are potential bioactives to counteract C. parvum infection in children. |
| |
Keywords: | OEO Oregano Essential Oil CV Carvacrol HCT-8 Human colon adenocarcinoma PP1 Pyrazolopyrimidine 1 IC50 inhibitory concentration 50 Oregano Carvacrol Cryptosporidium HCT-8 Parasite Corresponding author at: 905 S Goodwin Ave., Urbana, IL 61801, USA. |
本文献已被 ScienceDirect 等数据库收录! |
|