Double mutations in eIF4E and eIFiso4E confer recessive resistance to <Emphasis Type="Italic">Chilli veinal mottle virus</Emphasis> in pepper |
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Authors: | JeeNa Hwang Jinjie Li Wing-Yee Liu Song-Ji An Hwajin Cho Nam Han Her Inhwa Yeam Dosun Kim Byoung-Cheorl Kang |
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Institution: | (1) Department of Plant Breeding and Genetics, Cornell University, Ithaca, NY, USA;(2) Department of Ecology and Evolutionary Biology, Cornell University, Ithaca, NY, USA;(3) United States Department of Agriculture-Agricultural Research Station (USDA-ARS), Department of Plant Pathology, Cornell University, Ithaca, NY, USA;(4) College of Agricultural and Life Sciences, University of Wisconsin, Madison, WI, USA |
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Abstract: | To evaluate the involvement of translation initiation factors eIF4E and eIFiso4E in Chilli veinai mottle virus (ChiVMV) infection in pepper, we conducted a genetic analysis using a segregating population derived from a cross between
Capsicum annuum ‘Dempsey’ containing an eIF4E mutation (pvr1
2
) and C. annuum ‘Perennial’ containing an eIFiso4E mutation (pvr6). C. annuum ‘Dempsey’ was susceptible and C. annuum ‘Perennial’ was resistant to ChiVMV. All F1 plants showed resistance, and F2 individuals segregated in a resistant-susceptible ratio of 166:21, indicating that many resistance loci were involved. Seventy-five
F2 and 329 F3 plants of 17 families were genotyped with pvr1
2
and pvr6 allele-specific markers, and the genotype data were compared with observed resistance to viral infection. All plants containing
homozygous genotypes of both pvr1
2
and pvr6 were resistant to ChiVMV, demonstrating that simultaneous mutations in eIF4E and eIFiso4E confer resistance to ChiVMV in pepper. Genotype analysis of F2 plants revealed that all plants containing homozygous genotypes of both pvr1
2
and pvr6 showed resistance to ChiVMV. In protein-protein interaction experiments, ChiVMV viral genome-linked protein (VPg) interacted
with both eIF4E and eIFiso4E. Silencing of eIF4E and eIFiso4E in the VIGS experiment showed reduction in ChiVMV accumulation. These results demonstrated that ChiVMV can use both eIF4E
and eIFiso4E for replication, making simultaneous mutations in eIF4E and eIFiso4E necessary to prevent ChiVMV infection in pepper.
These authors contributed equally to this work. |
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Keywords: | eIF4E eIFiso4E host factor pepper recessive virus resistance |
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