Characterization of raffinose synthase from rice (Oryza sativa L. var. Nipponbare) |
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| Authors: | Suhong Li Tuoping Li Wook-Dong Kim Motomitsu Kitaoka Shigeki Yoshida Mitsutoshi Nakajima Hideyuki Kobayashi |
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| Affiliation: | (1) Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan;(2) Department of Food Science and Technology, Tianjing agricultural University, Tianjing, 300384, China;(3) Food Biotechnology Division, National Food Research Institute, National Agriculture and Food research Organization, Tsukuba, Ibaraki 305-8642, Japan;(4) Institute of Applied Biochemistry, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan;(5) Food Engineering Division, National Food Research Institute, National Agriculture and Food research Organization, Tsukuba, Ibaraki 305-8642, Japan |
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| Abstract: | The putative raffinose synthase gene from rice was cloned and expressed in Escherichia coli. The enzyme displayed an optimum activity at 45°C and pH 7.0, and a sulfhydryl group was required for its activity. The enzyme was specific for galactinol and p-nitrophenyl-α-d-galactoside as galactosyl donors, and sucrose, lactose, 4−β-galactobiose, N-acetyl-d-lactosamine, trehalose and lacto-N-biose were recognized as galactosyl acceptors. |
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| Keywords: | Raffinose synthase Substrate specificity Rice (Oryza sativa L. var. Nipponbare) Acceptor |
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