组织蛋白酶D和nm23基因蛋白在乳腺癌中的表达及与淋巴结转移的关系

采用免疫组织化学 ABC法检测了 43例乳腺癌中的组织蛋白酶 D和 nm 2 3基因蛋白的表达。结果显示组织蛋白酶D在乳腺癌组织中表达阳性率为 76 .7% (33/43)。 43例乳腺癌中 2 5例伴有淋巴结转移 ,18例无淋巴结转移 ,其组织蛋白酶D表达阳性率分别为 92 % (2 3/2 5 )及 5 5 .6 % (10 /18) ,两者之间具有显著性差异 (P<0 .0 1)。 nm 2 3基因蛋白在 43例乳腺癌中阳性率为 72 .1% (31/43)。在有淋巴结转移和无淋巴结转移的乳腺癌中 nm2 3基因蛋白阳性率分别为 6 0 % (15 /2 5 )及80 .9% (16 /18) ,nm2 3基因蛋白的表达与淋巴结转移呈显著负相关 (P<0 .0 5 )。结果提示对乳腺癌根治术后无淋巴结转移 ,但组织蛋白酶 D表达阳性 ,nm2 3基因蛋白表达阴性的患者 ,可能具有潜在的复发和转移倾向 ,应多加关注 ,并密切随访。

EXPRESSIONOF CATHEPSIN D AND nm23 PROTEIN AND RELATIONSHIP BETWEEN THEIREXPRESSION AND METASTASIS OF LYMPH NODE IN BREAST CANCER

The immunohistochemical ABC method was used to examine the expression of cathepsin D and nm23 protein in 43 cases of breast cancer The results showed that the positive rate of cathepsin D expression in breast cancers was 33/43(76 7%) The lymph node metastasis was 25 cases in 43 breast cancers The positive rates of cathepsin D expresson were 92% (23/25) and 55 6%(10/18) respectively in metastasis and non metastasis groups with significant difference ( P <0 01) The total positive rate of nm23 protein was 72 1% (31/43) in breast cancers in which the positive rate of nm23 protein was 60% (15/25) in lymph node metastasis group whihle 80 9% (16/18) in lymph node negative breast cancers The expression of nm23 protein in lymph node metastasis group was significantly lower than that in lymph node negative ones ( P <0 05) These results suggested that the careful follow up was necessary in cathepsin D positive and/or nm23 protein negative cases of the lymph node negative breast cancers because of the probability of recurrence ;