Genome Sizes of Desulfovibrio desulfuricans, Desulfovibrio vulgaris, and Desulfobulbus propionicus Estimated by Pulsed-Field Gel Electrophoresis of Linearized Chromosomal DNA |
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| Authors: | Richard Devereux Stephanie G Willis Mark E Hines |
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| Institution: | (1) United States Environmental Protection Agency, National Health and Environmental Effects Research Laboratory, Gulf Ecology Division, 1 Sabine Island Dr., Gulf Breeze, FL 32561, USA , US;(2) University of New Hampshire, Institute for the Study of Earth Oceans and Space, Durham, NH, USA , US |
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| Abstract: | Pulsed-field gel electrophoresis (PFGE) of linearized,
full-length chromosomal DNA was used to estimate the genome sizes of three
species of sulfate-reducing bacteria. Genome sizes of Desulfovibrio
desulfuricans, Desulfovibrio vulgaris, and Desulfobulbus
propionicus were estimated to be 3.1, 3.6, and 3.7 Mb, respectively.
These values are double the genome sizes previously determined for two
Desulfovibrio species by two-dimensional agarose gel electrophoresis
of DNA cut with restriction enzymes. PFGE of full-length chromosomal DNA
could provide a generally applicable method to rapidly determine bacterial
genome size and organization.
Received: 1 October 1996 / Accepted: 5 November 1996 |
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