Isolation and characterization of recombinant OmpF-like porin from the Yersinia pseudotuberculosis outer membrane |
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| Authors: | V A Khomenko O Yu Portnyagina O D Novikova M P Isaeva N Yu Kim G N Likhatskaya O P Vostrikova T F Solov’eva |
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| Institution: | (1) Pacific Institute of Bioorganic Chemistry, Far East Division, Russian Academy of Sciences, pr. Stoletiya Vladivostoka 159, Vladivostok, 690022, Russia |
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| Abstract: | The encoding sequence of the pore-forming OmpF-like protein from the Yersinia pseudotuberculosis outer membrane was cloned and expressed in Escherichia coli cells. Conditions for isolation and refolding of recombinant monomer and porin trimer were selected. Their spatial structures were characterized by the intrinsic protein fluorescence and CD spectroscopy. It was shown that recombinant porins are similar in the composition of secondary structure elements to isolated porins, but have a considerably less compact tertiary structure. The pore-forming activities of the recombinant proteins are similar to those of Y. pseudotuberculosis native porins. |
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| Keywords: | outer membrane recombinant porin refolding Yersinia pseudotuberculosis |
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