Overexpression of bacterioferritin comigratory protein (Bcp) enhance viability and reduced glutathione level in the fission yeast under stress |
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Authors: | Ga-Young Kang Eun-Hee Park Kyunghoon Kim Chang-Jin Lim |
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Institution: | (1) Division of Life Sciences and Research Institute of Life Sciences, Kangwon National University, Chuncheon, 200-701, Republic of Korea;(2) College of Pharmacy, Sookmyung Women’s University, Seoul, 140-742, Republic of Korea;(3) Korea Basic Science Institute, Chuncheon Center, Chuncheon, 200-701, Republic of Korea |
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Abstract: | The structural gene encoding bacterioferritin comigratory protein (Bcp) was amplified using PCR from the genomic DNA of Schizosaccharomyces pombe, and transferred into the shuttle vector pRS316 to generate the recombinant plasmid pBCPlO. The bcp
+ mRNA level in the pBCPlO-containing yeast cells was significantly higher than that in the control yeast cells, indicating
that the cloned gene is functioning. The S. pombe cells harboring the plasmid pBCPIO exhibited higher survival on the solid minimal media with hydrogen peroxide, tert-BOOH or cadmium than the control yeast cells. They also exhibited enhanced cellular viability in the liquid media containing
the stressful agents. The increased viabilities of the fission yeast cells harboring the plasmid pBCP10 were also obtained
with 0.4% glucose or 0.4% sucrose as a sole carbon source, and nitrogen starvation, compared with those of the control yeast
cells. The total glutathione (GSH) content and total GSH/GSSG ratio were significantly higher in the yeast cells harboring
the plasmid pBCP10 than in the control yeast cells. In brief, the S. pombe Bcp plays a protective role in the defensive response to oxidative stress possibly via up-regulation of total and reduced
glutathione levels. |
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Keywords: | fission yeast bacterioferritin comigratory protein glutathione peroxiredoxin Schizosaccharomyces pombe stress |
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