Auxotrophy of Pseudomonas aeruginosa in cystic fibrosis |
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Authors: | Rowena F.H. Taylor Margaret E. Hodson Tyrone L. Pitt |
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Affiliation: | Royal Brompton and National Heart Hospital, London, UK. |
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Abstract: | Seventy-four of 403 (18.4%) sputum isolates of Pseudomonas aeruginosa from 49 of 136 (36.0%) adults with cystic fibrosis (CF) were auxotrophic mutants. Two of 11 (18.2%) isolates of P. aeruginosa taken from patients with non-CF bronchiectasis were also auxotrophic. All 99 strains taken from non-bronchiectatic sources were prototrophic. Forty-six of 55 (83.6%) CF auxotrophs required one or more of 36 growth factors tested; the requirements for the remaining 9 isolates were not identified. Methionine was the sole factor required by 17 of 22 (77.3%) isolated which depended on a single factor. We conclude that auxotrophy is a feature of P. aeruginosa infection in cystic fibrosis. |
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Keywords: | Auxotrophy Pseudomonas aeruginosa Cystic fibrosis |
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