| pcDNA3．1表达载体转染对细胞生长的影响 |
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| 引用本文： | 余鹰,周鸣,曹利,唐珂,李伟芳,李桂源. pcDNA3．1表达载体转染对细胞生长的影响[J]. 生物技术, 2001, 11(1): 1-3 |
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| 作者姓名： | 余鹰 周鸣 曹利 唐珂 李伟芳 李桂源 |
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| 作者单位： | 湖南医科大学肿瘤研究所 |
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| 基金项目： | 国家高科技"863”项目资助(102-10-01-05);国家"973”重点项目资助;国家自然科学基金项目资助(39700158). |
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| 摘 要： | 为了探讨真核表达载体转染对细胞生长的影响,通过脂质体介导将pcDNA3.1( )表达载体DNA转染鼻咽癌细胞系HNE1,G418筛选后,Southern杂交鉴定稳定表达细胞株,以HNE1细胞为对照,观察pcDNA3.1( ）／HNE1克隆细胞的生物学特性;结果显示,在pcDNA3.1( )/HNE1阳性克隆中,一株细胞克隆培养过程中发生自溶性死亡,一株细胞生长明显受到抑制,另一株细胞生长无明显影响,揭示在宿主细胞中pcDNA3.1( )DNA与宿主基因组DNA发生了随机整合,从而表现不同的细胞生物学改变。
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| 关 键 词： | 真核表达系统 PCDNA3.1（＋）载体 整合 哺乳动物细胞 |
| 文章编号： | 1004-311(2001)01-0001-03 |
| 修稿时间： | 2000-09-01 |
Effects of pcDNA3.1 expression vector transfection on cell growth |
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| YU Ying,ZHOU Ming,CAO Li,TANG Ke,LI Wei - fang,LI Gui - yuan. Effects of pcDNA3.1 expression vector transfection on cell growth[J]. Biotechnology, 2001, 11(1): 1-3 |
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| Authors: | YU Ying ZHOU Ming CAO Li TANG Ke LI Wei - fang LI Gui - yuan |
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| Abstract: | pcDNA3 .1 expression vector is very popular for researcher to study gene function. In this paper, pcDNA3 .1 ( ) expression vector has been used to investigate its effects on HNE1 cell line by lipid transfection, the cytobiology of pcDNA3. 1 ( )/HNE1 cell line were analysised. Among the pcDNA3. 1 (-)/HNE1 positive clones detected, one was died autolytically in the process of cell culture; one grew slower than HNE1 ,the other had no evident change. This results implied pcDNA3. 1 ( ) plasmid DNA had integrated randomly with the chromosomes of host cell, and different biological effects appeared. |
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| Keywords: | eukaryon expression system pcDNA3.1( ) vector integration |
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