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Internal Transcribed Spacer (ITS) of rDNA of appendaged and non-appendaged strains of Microsporum gypseum reveals Microsporum appendiculatum as its synonym
Authors:Rahul Sharma  Ram C Rajak  Akhilesh K Pandey  Yvonne Gräser
Institution:(1) Mycological Research Laboratory, Department of Biological Sciences, Rani Durgavati University, Jabalpur, 482001, MP, India;(2) Institute of Microbiology and Hygiene, Department of Dermatology, Humboldt University, Berlin, Dorotheenstr, 96, Germany
Abstract:Recently a new taxon of geophilic dermatophytes was established as Microsporum appendiculatum Bhat and Mariam, based on the presence of appendaged macroconidia. However, such appendages are already known in the related species Microsporum gypseum. We conducted a survey of soil in central India as a part of a microbial biodiversity project and obtained two strains of M. gypseum with appendaged macroconidia. Using phenotypical characterization in combination with sequencing and restriction fragment length polymorphism (RFLP) of the Internal Transcribed Spacer (ITS) region of rDNA, we found that all strains of appendaged species are identical. Therefore M. appendiculatum is regarded as a synonym of M. gypseum.
Keywords:Microsporum appendiculatum            Conidial appendages            Microsporum gypseum            ITS-sequence analysis  Synonymy
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