Surfactant Effects on Lipase-Catalyzed Hydrolysis of Olive Oil in AOT/ISOOCTANE Reverse Micelles |
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| Authors: | Shau-Wei Tsai Kung-Ping Lee Chen-Li Chiang |
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| Affiliation: | a Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan, ROCb Department of Chemical Engineering, Nan-Tai College, Tainan, Taiwan, ROC |
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| Abstract: | The effects of surfactant concentration on the hydrolytic activity of Candida rugosa lipase in AOT/isooctane reverse micelles with olive oil as the substrate has been investigated. A noncompetitive inhibition by the surfactant on the enzyme was observed. Strong dependences of the kinetic constants kcat and kM, but not kI on the water-to-surfactant ratio (R value) have been identified. The benefits of carrying out the hydrolysis at higher surfactant and water concentrations were demonstrated from the improvement of the initial rate and time course of conversion. |
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| Keywords: | Reverse micelles lipase hydrolysis |
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