首页 | 本学科首页   官方微博 | 高级检索  
   检索      


Retrotransposons and siRNA have a role in the evolution of desiccation tolerance leading to resurrection of the plant Craterostigma plantagineum
Authors:Hilbricht Tobias  Varotto Serena  Sgaramella Vittorio  Bartels Dorothea  Salamini Francesco  Furini Antonella
Institution:Max-Planck-Institut fur Züchtungsforschung, Carl-von-Linne Weg 10, D-50829 Köln, Germany;;Dipartimento di Agronomia Ambientale e Produzione Vegetale, University of Padova, Agripolis-Viale dell'Università16, 35020 Legnaro, Padova, Italy;;Fondazione Parco Tecnologico Padano, CERSA, via Einstein –LocalitàCascina Codazza, 26900 Lodi, Italy;;Molekulare Physiologie und Biotecnologie der Pflanzen, University of Bonn, Kirschallee 1, D-53115 Bonn, Germany;;Dipartimento Scientifico e Tecnologico, University of Verona, Strada Le Grazie 15, 37134 Verona, Italy
Abstract:* Craterostigma plantagineum can lose up to 96% of its water content but fully recover within hours after rehydration. The callus tissue of the plant becomes desiccation tolerant upon pre-incubation with abscisic acid (ABA). In callus and vegetative organs, ABA addition and water depletion induce a set of dehydration-responsive genes. * Previously, activation tagging led to the isolation of Craterostigma desiccation tolerant (CDT-1), a dehydration-related ABA-inducible gene which renders callus desiccation tolerant without ABA pre-treatment. This gene belongs to a family of retroelements, members of which are inducible by dehydration. * Craterostigma plantagineum transformation with mutated versions of CDT-1 indicated that protein is not required for the induction of callus desiccation tolerance. Northern analysis and protoplast transfection indicated that CDT-1 directs the synthesis of a double-stranded 21-bp short interfering RNA (siRNA), which opens the metabolic pathway for desiccation tolerance. * Via transposition, these retroelements have progressively increased the capacity of the species to synthesize siRNA and thus recover after dehydration. This may be a case of evolution towards the acquisition of a new trait, stimulated by the environment acting directly on intra-genomic DNA replication.
Keywords:abscisic acid (ABA)                Craterostigma plantagineum              desiccation tolerance  retrotransposon  siRNA
本文献已被 PubMed 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号