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Quantitative Detection of Listeria monocytogenes in Biofilms by Real-Time PCR |
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| Authors: | Morgan Guilbaud Pierre de Coppet Fabrice Bourion Cinta Rachman Hervé Prévost and Xavier Dousset |
| Abstract: | A quantitative method based on a real-time PCR assay to enumerate Listeria monocytogenes in biofilms was developed. The specificity for L. monocytogenes of primers targeting the listeriolysin gene was demonstrated using a SYBR Green I real-time PCR assay. The number of L. monocytogenes detected growing in biofilms was 6 × 102 CFU/cm2. |
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