Structural homology of endosperm high molecular weight glutenin subunits of common wheat (Triticum aestivum L.) |
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Authors: | G Galili M Feldman |
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Institution: | (1) Department of Plant Genetics, The Weizmann Institute of Science, Rehovot, Israel |
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Abstract: | Summary Several high molecular weight endosperm glutenin subunits, coded by genes located on chromosomes 1A, 1B and 1D of common wheat,
Triticum aestivum L. em. Thell., were isolated from excised gel segments and subjected to amino acid analysis and peptide mapping; the latter
was carried out following a limited digestion with trypsin, chymotrypsin or Staphylococcus aureus — V8 protease. Generally, all high molecular weight glutenins had a similar amino acid composition but several significant
differences were observed in some of them. Both analyses revealed that the structural similarity among the various subunits
was related to the homology of the genes coding them: subunits coded by homoalleles, i.e., different alleles of the same gene,
were most similar; those coded by homoeoalleles, i.e., alleles of homoeologous genes, were less similar; whereas subunits
coded either by alleles of different genes of the same gene cluster, or by nonhomoeoalleles of homoeologous clusters, were
the least similar. Several small peptides derived from protease digestion of various subunits had a higher than expected staining
intensity indicating that small peptide repeats may be interspersed within the glutenin subunits. The evolutionary course
of the high molecular weight glutenins is discussed. |
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Keywords: | Hexaploid wheat Triticum aestivum Glutenins Electrophoresis Amino acid analysis Peptide mapping |
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