交联I型胶原复合生物玻璃在兔股骨远端缺损中的成骨作用

摘要 目的：评价交联度不同的I型胶原复合生物玻璃后作为人工骨移植物在兔股骨髁部骨缺损中的修复作用，以研究一种成骨性能优秀，降解速度令人满意，且具有可塑性,便于术中使用的新型人工骨移植材料。方法：本研究设置实验组及对照组，实验组为交联度70%的高交联I型胶原复合生物玻璃以及交联度为45%的低交联I型胶原复合生物玻璃。对照组为普通未交联I型胶原复合生物玻璃。于9只新西兰大白兔双下肢股骨髁部制备动物骨缺损模型，将随机分组后的三种骨移植物分别植入股骨髁部骨缺损模型中。术后6周取材行组织学分析研究，比较3种骨移植物在骨缺损中的新骨生成率。结果：组织学分析结果显示，高交联组的新骨生成率为5.23 0.87%，其成骨性能显著低于低交联组13.23 1.13%以及未交联组的12.63 0.92%（P<0.05）。而低交联组的新骨生成率与未交联组之间无统计学差异（P>0.05）。结论：交联度为45%的低交联I型胶原复合生物玻璃具有更好的成骨能力，作为骨移植材料在临床应用中具有更广阔的发展前景。

Effect of the Cross Linking Collagen Type I/Bioglass Composite Repair the Distal Femur Defect in Rabbit

ABSTRACT Objective: To evaluate thebone formation of the artificial bone grafts which fabricated by type I collagen with different crosslinking degree composite with bioglass, in the distal femur defect model in rabbits. To develop a new type plastic artificial bone graft with excellent bone formation performance and slow degradation rate. Methods: High-cross linking collagen(crosslinking degree 70%) composite with bioglass, Low-cross linking collagen (crosslinking degree 45%) composite with bioglass and Non-cross linking collagen composite with bioglass have been implanted into the lower limbsdistal femur defects of 9 New Zealand white rabbits. The samples have been harvested at 6 weeks after the operation. The histological examination has been done to evaluate the new bone formation ratio in bone defect model of the rabbits, which implanted with the three kinds of artificial bone grafts. Results: The histological analysis showed thatthe newly bone formation ratio of the High-cross linking group is 5.23 0.87%, the newly bone formation ratio of theLow-crosslinking group is 13.23 1.13%, the newly bone formation ratio of theNon-crosslinking group is 12.63 0.92%. The rate of new bone formation in the High-cross linking group is significantly lower than that in theLow-crosslinking group and the Non-crosslinking group (P<0.05). However, the newly bone formation ratio there is no statistical difference between in the Low-crosslinking group and the Non-crosslinking group (P>0.05). Conclusion: The research revealed that the Low-cross linking collagen possess excellent osteogenic properties, and suitablefor composite with bioglassas artificial bone grafts.