Immunohistochemical localization of rainbow trout ladderlectin and intelectin in healthy and infected rainbow trout (Oncorhynchus mykiss) |
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| Authors: | S. Russell M.A. Hayes J.S. Lumsden |
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| Affiliation: | 1. Department of Medicine and Cardiology, Academic Teaching Hospital Feldkirch, Feldkirch, Austria;2. Vorarlberg Institute for Vascular Investigation and Treatment (VIVIT), Feldkirch, Austria;3. Private University of the Principality of Liechtenstein, Triesen, Liechtenstein;4. Drexel College University of Medicine, Philadelphia, PA, USA;1. Department of Biochemistry, University of Wisconsin-Madison, 433 Babcock Drive, 53706, USA;2. Department of Chemistry, University of Wisconsin-Madison, 1101 University Avenue, 53706, USA;3. Center for Genome Sciences and Systems Biology, Washington University School of Medicine, St. Louis, MO 63110, USA |
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| Abstract: | In the present study, the pattern of immuno-reactive ladderlectin and intelectin in healthy rainbow trout is compared to rainbow trout infected with a variety of infectious agents. In healthy rainbow trout, both proteins were localized to individual epithelial cells of the gill and intestine and both proteins were clearly demonstrated within cytoplasmic granules of polymorphonuclear leucocytes and macrophages/monocytes found in blood vessels, hepatic sinusoids, renal interstitium, mucosal epithelium and submucosa of normal intestine. In tissue from infected rainbow trout, there was an overall relative increase in both lectins compared to healthy fish and both proteins were detected in extra-cellular spaces surrounding bacteria, fungi and protozoa. Increased distribution and density of both RTLL and RTInt was demonstrated along mucosal surfaces and within inflammatory leucocytes in infected tissues and immune related organs. These findings represent one of the few examples of in vivo association of defence lectins and infectious agents. |
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