Immune responses,ROS generation and the haemocyte damage of scallop Chlamys farreri exposed to Aroclor 1254 |
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| Authors: | Jing Liu Lu-Qing Pan Lin Zhang Jingjing Miao Jing Wang |
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| Institution: | 1. Ifremer, UMR 5244, IHPE Interaction Host Pathogen Environment, UPVD, CNRS, Université de Montpellier, CC 80, F-34095 Montpellier, France;2. Laboratory of Immunology Applied to Aquaculture, Department of Cell Biology, Embryology and Genetics, Federal University of Santa Catarina, 88040-900 Florianópolis, SC, Brazil;3. Laboratorio de Genética e Inmunología Molecular, Instituto de Biología, Pontificia Universidad, Católica de Valparaíso, Avenida Universidad 330, 2373223 Valparaíso, Chile;1. Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;2. University of Chinese Academy of Sciences, Beijing 100049, China;3. Dalian Ocean University, Dalian 116023, China;1. Qinzhou University, Qinzhou 535011, China;2. Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, South China Sea Resource Exploitation and Protection Collaborative Innovation Center (SCS-REPIC), Guangzhou 510300, China;3. South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China;4. College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China;1. Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;2. Functional Laboratory of Marine Fisheries Science and Food Production Process, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China;3. Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian 116023, China;4. University of Chinese Academy of Sciences, Beijing 100049, China;1. Centro de Investigacións Mariñas, Consellería do Medio Rural e do Mar, Xunta de Galicia, Aptdo. 13, 36620 Vilanova de Arousa, Spain;2. Eckerd College, St. Petersburg, FL 33701, USA |
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| Abstract: | The toxic effects of Aroclor 1254 (0.05, 0.5, 5 and 50 μg l?1) on scallop (Chlamys farreri) immune system in vivo were studied. The results showed that Aroclor 1254 had significant toxic effect on the parameters tested in this paper (P < 0.05). The total number of haemocytes, the proportion of granulocytes, phagocytosis in all groups as well as the lysosomal membrane stability (LMS) in 5, 50 μg l?1 and bacteriolytic activity 0.5, 5, 50 μg l?1 treatments decreased significantly, while the proportion of hyalinocytes and the production of O2- in all treatments remarkably increased during the sampling time and tended to be stable gradually after 6–15 d. The bacteriolytic activity in 0.05 μg l?1 treatments, LMS in 0.05, 0.5 μg l?1 groups and the DNA damage (comet ratios and arbitrary values) in all treatments increased at the beginning of exposure and reached their peaks on day 1, day 1, day 6 and day 3, following that they all decreased gradually and became stable after 9–15 d. When the indices reached stability, except for DNA damage was higher than controls, the others were all significantly lower than those of controls (P < 0.05). Thus, Aroclor 1254 has evident toxic effects on scallop immune system, which supports the view that a relationship exists between pollution and immunomodulation in aquatic organisms. Also it supports the speculation that the PCBs pollution is one of the important reasons of the mass mortality of the C. farreri. |
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