Isolation and characterization of Toll-like receptor 9 in half-smooth tongue sole Cynoglossus semilaevis |
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| Authors: | Yan Yu Qiwang Zhong Chunmei Li Liming Jiang Fusheng Yan Zhigang Wang Quanqi Zhang |
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| Affiliation: | 1. Department of Urology, College of Medicine, Soonchunhyang University, Seoul Hospital, Seoul, South Korea;2. Department of Radiology, College of Medicine, Soonchunhyang University, Seoul Hospital, Seoul, South Korea;3. Medical Research Collaborating Center, Seoul National University Bundang Hospital, Seongnam, South Korea;1. Department of Urology, People''s Hospital of Xinjiang Uyghur Autonomous Region, Urumqi, Xinjiang, China;2. Department of Clinical Laboratory, People''s Hospital of Xinjiang Uyghur Autonomous Region, Urumqi, Xinjiang, China;3. Department of Urology First People''s Hospital of Kashi, Kashi, Xinjiang, China;1. Center for Vascular Biology, Department of Pathology and Laboratory Medicine, Cornell University, New York, NY;2. Department of Surgery, Weill Cornell Medical College, Cornell University, New York, NY;1. Fisheries Department, Faculty of Natural Resources, Urmia University, Urmia, Iran;2. Young Researchers and Elites Club, North Tehran Branch, Islamic Azad University, Tehran, Iran;3. Rajaei Sturgeon Hatchery Center, Sari, Iran;4. Fisheries Department, Faculty of Agriculture and Natural Resources, Persian Gulf University, Bushehr, Iran;5. Medicine Laboratory, Alavi Educational and Treatment Center, Ardabil University of Medical Sciences, Ardabil, Iran;6. Biotechnology Laboratory, Faculty of Agriculture and Natural Resources, Tehran University, Karaj, Iran;1. The Fish Molecular Genetics and Biotechnology Laboratory, Department of Fisheries and Allied Aquacultures and Program of Cell and Molecular Biosciences, Aquatic Genomics Unit, Auburn University, Auburn, AL 36849, USA;2. Central Institute of Fisheries Education (CIFE), Versova, Andheri (W), Mumbai 400061, India |
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| Abstract: | ![]()
Toll-like receptors (TLRs) are considered as key sensors to trigger the host's innate immune system and adaptive immune responses by recognizing various PAMPs and initiating signal transduction. TLR9, as a member of TLR family, mediates the recognition of unmethylated CpG dinucleotide motifs commonly found in both bacterial and viral genomes. In the current study, the TLR9 gene was isolated from one of flatfish species, half-smooth tongue sole (Cynoglossus semilaevis). In the 4588 bp genomic sequence, three exons, two introns, and 5′ UTR of 23 bp and 3′ UTR of 342 bp were identified. Putative amino acid sequence was 1062 residues long, including a typical conserved cytosolic Toll/interleukin-1 receptor (TIR) domain, 14 leucine-rich repeat (LRR) motifs, with greater than 60% identity to gilthead sea bream Sparus aurata and Japanese flounder Paralichthys olivaceus orthologs. Quantitative RT-PCR analysis indicated a broad expression of csTLR9, especially in spleen and gonads. No statistically significant changes were observed for csTLR9 mRNA levels in spleen and head kidney after inactive Vibrio anguillarum immunisation. In C. semilaevis ontogeny, the expression of csTLR9 appeared to be developmentally regulated. The presence of maternal TLR9 mRNA and the dramatic decrease of TLR9 expression at metamorphic stage indicated TLR9 might be involved in C. semilaevis development. Comparing sequence and expression profile of csTLR9 with mammalian and other piscine TLR9s suggested that the main function of TLR9 might be conserved across vertebrates, although species-specific features were present. |
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