Retronectin enhances lentivirus-mediated gene delivery into hematopoietic progenitor cells |
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Authors: | Hyun-Joo Lee Yong-Soo Lee Hye-Sun Kim Yu-Kyung Kim Jae-Hwan Kim Seong-Ho Jeon Hyeon-Woo Lee Sinae Kim Hiroyuki Miyoshi Hyung-Min Chung Dong-Ku Kim |
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Institution: | 1. Graduate School of Life Science and Biotechnology, Pochon CHA University, CHA Stem Cell Institute, 605 Yeoksam 1-dong, Kangnam-gu, Seoul 135-081, Republic of Korea;2. College of Pharmacy, Kangwon National University, Chuncheon 200-701, Republic of Korea;3. Department of Pharmacology, Institute of Oral Biology, School of Dentistry, Kyung Hee University, Seoul 130-701, Republic of Korea;4. Subteam for Manipulation of Cell Fate, BioResource Center, RIKEN Tsukuba Institute, Tsukuba, Ibaraki 305-0074, Japan |
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Abstract: | Genetic modification of hematopoietic stem cells holds great promise in the treatment of hematopoietic disorders. However, clinical application of gene delivery has been limited, in part, by low gene transfer efficiency. To overcome this problem, we investigated the effect of retronectin (RN) on lentiviral-mediated gene delivery into hematopoietic progenitor cells (HPCs) derived from bone marrow both in vitro and in vivo. RN has been shown to enhance transduction by promoting colocalization of lentivirus and target cells. We found that RN enhanced lentiviral transfer of the VENUS transgene into cultured c-Kit+ Lin? HPCs. As a complementary approach, in vivo gene delivery was performed by subjecting mice to intra-bone marrow injection of lentivirus or a mixture of RN and lentivirus. We found that co-injection with RN increased the number of VENUS-expressing c-Kit+ Lin? HPCs in bone marrow by 2-fold. Further analysis of VENUS expression in colony-forming cells from the bone marrow of these animals revealed that RN increased gene delivery among these cells by 4-fold. In conclusion, RN is effective in enhancing lentivirus-mediated gene delivery into HPCs. |
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