Serum disposition of bovine lactoferrin after oral and anal administration and its proteolytic cleavage by gastric transit in rainbow trout (Oncorhynchus mykiss W.) |
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| Authors: | Stefano Cecchini Anna R Caputo |
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| Institution: | 1. Dipartimento di Scienze delle Produzioni Animali, Università degli Studi della Basilicata, Campus Universitario di Macchia Romana, 85100 Potenza, Italy;2. Istituto Sperimentale per la Zootecnia, 85054 Bella Scalo, Potenza, Italy;1. Department of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran;2. School of Applied Science, Temasek Polytechnic, 529757, Singapore;1. Fish and Animal Production Department, Faculty of Agriculture (Saba Basha), Alexandria University, Egypt;2. Aquaculture Division, National Institute of Oceanography and Fisheries, Cairo, Egypt;3. Aquaculture Division, National Institute of Oceanography and Fisheries, Alexandria, Egypt;4. Fish Innate Immune System Group, Department of Cell Biology and Histology, Faculty of Biology, Regional Campus of International Excellence “Campus Mare Nostrum”, University of Murcia, 30100 Murcia, Spain;1. AQUOS Laboratory - Health of Aquatic Organisms, Department of Aquaculture, Center for Agricultural Sciences, Federal University of Santa Catarina (UFSC), Rod Admar Gonzaga 1346, 88040-900, Florianópolis, Santa Catarina, Brazil;2. Laboratory for Biomarkers of Aquatic Contamination and Immunochemistry, Department of Biochemistry, CCB, UFSC, Brazil;3. Laboratory of Plant Morphogenesis and Biochemistry, Department of Plant Science, CCA, UFSC, Brazil;1. Department of Fish Diseases and Management, Zagazig University, Zagazig, Sharkia, Egypt;2. Department of Nutrition and Clinical Nutrition, Zagazig University, Zagazig, Sharkia, Egypt;3. Department of Animal Wealth Development, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Sharkia, Egypt;1. Newcastle Neonatal Service, Newcastle upon Tyne Hospitals NHS Foundation Trust, Queen Victoria Road, Newcastle upon Tyne NE1 4LP, UK;2. Institute of Health and Society, Newcastle University, Newcastle upon Tyne, UK;3. National Institute for Health Research, Centre for Reviews and Dissemination, University of York, York, UK;4. School of Life Sciences, University of Northumbria, Newcastle upon Tyne, UK |
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| Abstract: | Several studies have shown an immunomodulatory effect of orally administered bovine lactoferrin (LF) in fish, but the process of digestion was not characterized.In the present study, we investigated the fate of bovine LF after oral and anal administration, and studied the appearance of intact LF in the bloodstream and its proteolytic attack during the gastric transit in rainbow trout (Oncorhynchus mykiss) held at 9 °C and 18 °C.Data obtained showed the presence of intact bovine LF in the bloodstream only after anal administration in fish held at 18 °C and the presence of several peptides derived from bovine LF in the gastric content. Immunoblotting analysis showed that only a part of bovine LF-derived peptides reacted with the applied anti-bovine LF antibody. The concentration of intact bovine LF, after 30 min of administration, in the gastric content of fish reared at 18 °C, being extremely low, if any, led us to suspect that the immunoregulatory effect of dietary bovine LF shown in fish by several authors is not due to the intact form but to bioactive fragments, originated by the proteolytic attack during the gastric transit, as demonstrated in higher vertebrates. |
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