| 芫花的花粉形态观察及生活力和萌发率分析 |
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| 引用本文： | 刘芳,汪甜,王杨,沈永宝.芫花的花粉形态观察及生活力和萌发率分析[J].植物资源与环境学报,2011,20(1):94-96. |
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| 作者姓名： | 刘芳 汪甜 王杨 沈永宝 |
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| 作者单位： | 南京林业大学,江苏南京,210037 |
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| 摘 要： | Pollen morphology of Daphne genkwa Sieb.et Zucc.was observed by scanning electron microscope(SEM),and its viability and germination rate were measured by TTC and sucrose in vitro culture methods.The results show that pollen is stenopalynous type with a diameter of 15.6-21.6 μm.Per pollen has 10-16 apertures which is irregular circular with a diameter of 1.4-2.0 μm.Surface ornamentation of pollen is rough reticulate pattern which is circular polygon(tetragon-heptagon,mostly pentagon-hexagon).Pollen viability is 51% by TTC.Sucrose of different concentrations has a significant effect on pollen germination rate during pollen culture.And pollen germination rate is the highest with a percentage of 27.0% in medium containing 50 g·L-1 sucrose,while pollen could not germinate in medium containing sucrose over 250 g·L-1.Otherwise,there is the phenomenon of "multi-aperture germination" during pollen germinating.
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| 关 键 词： | 芫花 花粉 形态特征 生活力 萌发率 |
Morphological observation and analyses of viability and germination rate of Daphne genkwa pollen |
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| LIU Fang,WANG Tian,WANG Yang,SHEN Yong-bao.Morphological observation and analyses of viability and germination rate of Daphne genkwa pollen[J].Journal of Plant Resources and Environment,2011,20(1):94-96. |
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| Authors: | LIU Fang WANG Tian WANG Yang SHEN Yong-bao |
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| Institution: | LIU Fang,WANG Tian,WANG Yang,SHEN Yong-bao(Nanjing Forestry University,Nanjing 210037,China) |
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| Abstract: | |
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| Keywords: | Daphne genkwa Sieb et Zucc pollen morphology viability germination rate |
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