| 萝卜带柄子叶高频再生体系的建立 |
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| 引用本文： | 李海萍,张鲁刚,张静,茹磊,刘学成,孙希禄. 萝卜带柄子叶高频再生体系的建立[J]. 植物学通报, 2011, 46(3): 331-337. DOI: 10.3724/SP.J.1259.2011.00331 |
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| 作者姓名： | 李海萍 张鲁刚 张静 茹磊 刘学成 孙希禄 |
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| 作者单位： | 西北农林科技大学园艺学院,杨陵,712100 |
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| 摘 要： | 以11份萝卜（Raphanus sativus）基因型为材料进行子叶离体培养研究,筛选出具有较高再生率的基因型进行实验,考察基因型、外植体类型、激素配比和苗龄等因素对萝卜再生的影响。结果表明：萝卜离体再生的最佳外植体为全子叶-叶柄,最适苗龄为4天,最适培养基为MS＋6mg·L-16-BA＋0.05mg·L-1NAA,再生率高达86.95%,再生系数为1.80。该研究为进行萝卜遗传转化实验奠定了良好基础。
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| 关 键 词： | 离体再生 萝卜 6-BA TDZ |
Establishment of High-frequency Regeneration System from Cotyledons with Petiole in Radish |
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| Haiping Li,Lugang Zhang,Jing Zhang,Lei Ru,Xuecheng Liu,Xilu Sun. Establishment of High-frequency Regeneration System from Cotyledons with Petiole in Radish[J]. Chinese Bulletin of Botany, 2011, 46(3): 331-337. DOI: 10.3724/SP.J.1259.2011.00331 |
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| Authors: | Haiping Li Lugang Zhang Jing Zhang Lei Ru Xuecheng Liu Xilu Sun |
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| Affiliation: | Haiping Li,Lugang Zhang,Jing Zhang,Lei Ru,Xuecheng Liu,Xilu Sun College of Horticulture,Northwest Agriculture and Forestry University,Yangling 712100,China |
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| Abstract: | We studied in vitro culture regeneration from cotyledons of 11 genotypes of radish.Those genotypes,which have a high rate of regeneration,were used as the test materials for subsequent experiments of explant type,hormone ratio,and seedling age.The regenerated plantlets,which generated roots well,were transplanted into the field.The entire cotyledon with a petiole is the optimal regeneration explant.Four days of seedling age is suitable to induce adventitious shoots.The maximum frequency of shoot regeneratio... |
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| Keywords: | 6-BA TDZ |
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