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中国白灵侧耳自然群体的交配型因子分析
引用本文:李何静,陈强,图力古尔,黄晨阳,赵梦然,张金霞.中国白灵侧耳自然群体的交配型因子分析[J].菌物学报,2013,32(2):248-252.
作者姓名:李何静  陈强  图力古尔  黄晨阳  赵梦然  张金霞
作者单位:1. 吉林农业大学 吉林长春130118;中国农业科学院农业资源与农业区划研究所 农业部农业微生物资源收集与保藏重点实验室 北京100081
2. 中国农业科学院农业资源与农业区划研究所 农业部农业微生物资源收集与保藏重点实验室 北京100081
3. 吉林农业大学 吉林长春130118
基金项目:现代农业产业技术体系建设专项资金(No. CARS-24)
摘    要:为了研究我国新疆白灵侧耳自然种群的遗传多样性,估测自然种群的遗传丰度,对51株新疆野生白灵侧耳的84个原生质体单核体的交配型因子进行分析。在84个单核体中存在54个不同的A因子和59个不同的B因子。x2检测表明交配型因子A和B的系列因子均为等概率分布。据此估算我国白灵侧耳自然群体中有79个A因子,100个B因子,自然群体中的单核交配型总数79×100=7,900个,双核交配型总数31,201,050个。可见,新疆白灵侧耳遗传多样性丰富,新疆是我国白灵侧耳的野生种质资源宝库。

关 键 词:种质资源  单核体  双核体  遗传多样性  对峙培养

Analysis of the mating type factors in natural populations of Pleurotus eryngii var. tuoliensis in China
Authors:LI He-Jing  CHEN Qiang  BAU Tolgor  HUANG Chen-Yang  ZHAO Meng-Ran and ZHANG Jin-Xia
Institution:Jilin Agricultural University, Jilin, Changchun 130118, China Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences; Key Laboratory of Microbial Resources, Ministry of Agriculture, Beijing 100081, China;Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences; Key Laboratory of Microbial Resources, Ministry of Agriculture, Beijing 100081, China;Jilin Agricultural University, Jilin, Changchun 130118, China;Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences; Key Laboratory of Microbial Resources, Ministry of Agriculture, Beijing 100081, China;Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences; Key Laboratory of Microbial Resources, Ministry of Agriculture, Beijing 100081, China;Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences; Key Laboratory of Microbial Resources, Ministry of Agriculture, Beijing 100081, China
Abstract:In order to investigate the genetic diversity and estimate the genetic abundance of natural populations of Pleurotus eryngii var. tuoliensis, mating type factors in 84 protoplast monokaryons derived from 51 isolations collected from Xinjiang were analyzed. There were 54 different A factors and 59 different B factors in the 84 monokaryons. Chi-square test indicated that A and B factors were distributed with equal probability. Therefore there would be 79 different A factors and 100 distinct B factors in natural populations of P. eryngii var. tuoliensis in Xinjiang, China, and the putative mating type factors from monokaryon are 7,900, and for dikaryon, there would be 31,201,050 in nature. In conclusion, genetic diversity of P. eryngii var. tuoliensis is very high in Xinjiang, and Xinjiang is a valuable wild P. eryngii var. tuoliensis germplasm resource pool.
Keywords:germplasm resource  monokaryon  dikaryon  genetic diversity  confrontation culture
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