Elicitor-mediated induction of isochorismate synthase and accumulation of 2,3-dihydroxy benzoic acid in Catharanthus roseus cell suspension and shoot cultures |
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Authors: | Paulo R. H. Moreno Robert van der Heijden Robert Verpoorte |
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Affiliation: | (1) Leiden/Amsterdam Centre for Drug Research, Division of Pharmacognosy, Gorlaeus Laboratoria, Leiden University, P.O. Box 9502, 2300 RA — Leiden, The Netherlands |
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Abstract: | After elicitation, cell suspension cultures of Catharanthus roseus accumulate phenolic compounds. The major phenolic compound produced was isolated and identified as 2,3-dihydroxybenzoic acid (DHBA). The accumulation of this compound is a rapid response to the addition of elicitor; within 6 h after the addition of elicitor, DHBA concentration reached 6.3 mg/l cell suspension. DHBA was not detected in non-elicited cells. The formation of DHBA in elicited cells was correlated with the induction of the enzyme isochorismate synthase (ICS). Shoot cultures of C. roseus also presented a strong induction of ICS after elicitation. Due to its biological activity, DHBA could play a role in the defence mechanism of C. roseus. |
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