An engineered Bacillus thuringiensis strain with insecticidal activity against Scarabaeidae (Anomala corpulenta) and Chrysomelidae (Leptinotarsa decemlineata and Colaphellus bowringi) |
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| Authors: | Ginxin Yan Fuping Song Changlong Shu Jingjing Liu Chunqin Liu Dafang Huang Shuliang Feng Jie Zhang |
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| Affiliation: | (1) State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences (CAAS), 100193 Beijing, China;(2) Cangzhou Academy of Agricultural and Forestry Sciences, 061001 Cangzhou, China;(3) Biotechnology Research Institute, CAAS, 100081 Beijing, China;(4) Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, 071000 Baoding, China |
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| Abstract: | A genetically-engineered Bacillus thuringiensis (Bt) strain, 3A-HBF, with a broad insecticidal spectrum was constructed by introducing the recombinant plasmid pSTK-3A containing cry3Aa7 into the wild-type Bt strain HBF-1 containing the cry8Ca2 gene. The Cry3Aa7 protein produced by strain 3A-HBF was verified by SDS-PAGE and Western blotting. Flat rectangular crystals of Cry3Aa7 protein were observed besides spherical crystals (Cry8Ca2). The plasmid pSTK-3A was stable when strain 3A-HBF was grown in medium without antibiotics. The growth rate of 3A-HBF was not significantly different from that of the recipient strain, HBF-1. Strain 3A-HBF showed toxicity against two families of pests, Scarabaeidae and Chrysomelidae pests, which are susceptible to Cry8Ca (Anomala corpulenta) and Cry3Aa (Leptinotarsa decemlineata and Colaphellus bowringi). The 50% lethal concentrations of 3A-HBF against A. corpulenta, L. decemlineata and C. bowringi were 0.730 × 108 c.f.u./g dry soil, 1.74 μg/ml and 1.15 μg/ml, respectively. |
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| Keywords: | Bacillus thuringiensis Chrysomelidae Insecticidal activity Scarabaeidae |
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