Mice immunization with live lactococci displaying a surface anchored HPV-16 E7 oncoprotein |
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| Authors: | Cortes-Perez Naima G Bermúdez-Humarán Luis G Le Loir Yves Rodriguez-Padilla Cristina Gruss Alexandra Saucedo-Cárdenas Odila Langella Philippe Montes-de-Oca-Luna Roberto |
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| Institution: | Laboratorio de Inmunología y Virología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolás de los Garza, N.L. 66450, Mexico. |
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| Abstract: | E7 oncoprotein of human papillomavirus-16 (HPV-16) is constitutively produced in cervical cancer (CxCa) and is a good candidate for the design of therapeutic vaccines. In this work, the nisin-controlled expression system was used to display the E7 protein at the cell surface of the food-grade Gram-positive bacterium Lactococcus lactis. An efficient cell wall anchoring of E7 was obtained. Intranasal administration of these recombinant lactococci in mice induced an HPV-16 E7-specific immune response. This is the first report of E7 cell wall anchoring in L. lactis and represents one more step towards the use of live food-grade bacteria to fight against CxCa. |
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| Keywords: | Lactococcus lactis Cancer Human papillomavirus Vaccine Immunogenic |
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