Photosynthesis and carbon metabolism in plantain (Musa AAB) plantlets growing in temporary immersion bioreactors and during ex vitro acclimatization |
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Authors: | Carlos E Aragón Maritza Escalona Iris Capote Danilo Pina Inaudis Cejas Roberto Rodriguez Maria Jesus Cañal Jorge Sandoval Sophe Roels Pierre Debergh Justo Gonzalez-Olmedo |
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Institution: | (1) Laboratory for Plant Cell and Tissue Culture, Bioplant Centre, University of Ciego de Avila, 69450 Cuba;(2) Dept. Biología y Organismos de Sistemas, University of Oviedo, Spain;(3) CORBANA, Costa Rica;(4) Department of Plant Production, University of Gent, Belgium |
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Abstract: | Summary The photosynthetic capacity changes and the main enzymatic systems related to carbon metabolism were investigated during the
in vitro culture of plantain shoots (Musa AAB cv. CEMSA 3/4) in temporary immersion bioreactors (TIB) and their subsequent acclimatization.
The maximal rate of photosynthesis (Pn), transpiration, and the activity of the carbon metabolism enzymes phosphoenolpyruvate
carboxylase (PEPC), acid invertase (AI), pyruvate kinase (PK) and sucrose phosphate synthase (SPS) were measured every 7 d
during the 21 d of elongation in TIB, and the following 42 d of acclimatization. Sucrose content in the liquid medium and
in the leaves was also determined. The most significant changes in plant growth were observed during acclimatization. During
the in vitro stage photosynthesis was limited (4–6 μmol CO2m−2s−1); the photosynthetic rate however increases rapidly and significantly as soon as in vitro culture is over during acclimatization. PEPC activity increased during the whole evaluation period. The highest levels were
achieved around days 42 and 56. PK and SPS activities were optimal during the first weeks in acclimatization (28–35 d), while
AI increased at the beginning of the elongation phase (7 d), and later at the end of the acclimatization (49–63 d). The relationships
between morphological parameters, photosynthetic capacity of the plantlets and the carbon metabolism enzymes during both phases
of the culture are discussed. |
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Keywords: | enzymes liquid medium micropropagation physiology |
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