Ca2+ release induced by cyclic ADP ribose in mice lacking type 3 ryanodine receptor. |
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| Authors: | R Fulceri R Rossi R Bottinelli A Conti E Intravaia A Galione A Benedetti V Sorrentino C Reggiani |
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| Institution: | Department of Physio-Pathology and Experimental Medicine, University of Siena, Italy. |
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| Abstract: | The action of cyclic-ADP-ribose was studied on calcium release from sarcoplasmic reticulum of skeletal muscles of neonatal and adult wild-type and RyR3-deficient mice. cADPR increased calcium efflux from microsomes, enhanced caffeine-induced calcium release, and, in 20% of the tests, triggered calcium release in single muscle fibers. These responses occurred only in the diaphragm of adult RyR3-deficient mice. cADPR action was abolished by ryanodine, ruthenium red, and 8-brome-cADPR. These results strongly favor a specific action of cADPR on RyR1. The responsiveness of RyR1 appears in adult muscles when RyR3 is lacking. |
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