| 阴道分离因肯毛孢子菌的分子鉴定和种内分型 |
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| 引用本文： | 吕雪莲,张晓利,王淼淼,佘晓东,吕桂霞,沈永年,刘维达.阴道分离因肯毛孢子菌的分子鉴定和种内分型[J].菌物学报,2010,29(1):91-95. |
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| 作者姓名： | 吕雪莲 张晓利 王淼淼 佘晓东 吕桂霞 沈永年 刘维达 |
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| 作者单位： | 中国医学科学院皮肤病研究所,南京,210042 |
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| 基金项目： | 科技重大专项（No. 2008ZX10004-002）；卫生部部属（管）医院临床学科重点项目（No. 2007.7）；卫生部公益性行业科研专项经费项目（No. 200802032）；江苏省自然科学基金（No. BK2008091） |
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| 摘 要： | 分别采用rRNA基因内转录间隔区(ITS)和基因间隔区(IGS1)测序,ITS和IGS1区PCR限制性片段长度多态性分析(PCR-RFLP)和基因组DNA的随机扩增多态性DNA(RAPD)等方法,对三株因肯毛孢子菌Trichosporon inkin进行分子特征及种内分型研究。结果显示,不同菌株的rRNA基因ITS区和IGS1区的序列相似性均高达100%,RFLP酶切图谱具有较理想的种内一致性,而不同菌株的RAPD图谱不尽相同。研究表明:rRNA基因IGS1区测序及RFLP酶切可考虑用于因肯毛孢子菌的菌种分子鉴定,而基因组DNA的RAPD则较适合于菌种的种内分型。
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| 关 键 词： | rRNA基因测序 PCR限制性片段长度多态性分析 随机扩增多态性DNA |
Molecular identification and genotyping of Trichosporon inkin isolated from vagina |
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| Authors: | LU Xue-Lian ZHANG Xiao-Li WANG Miao-Miao SHE Xiao-Dong LU Gui-Xia SHEN Yong-Nian LIU Wei-Da |
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| Institution: | Institute of Dermatology, Chinese Academy of Medical Sciences, Nanjing 210042, China |
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| Abstract: | Three Trichosporon inkin strains isolated from different sources were characterized by sequencing and PCR-restriction fragment length polymorphism (PCR-RFLP) analyses of the internal transcribed spacer (ITS) and the intergenic spacer 1 (IGS1) regions of rRNA genes, and random amplified polymorphic DNA (RAPD). The ITS and IGS1 sequences of the T. inkin strains studied were identical. Their restriction fragment patterns of ITS and IGS1 were also indistinguishable. The RAPD patterns of the 3 strains amplified by primer M13, OPA4 and OPA13 displayed some degree of intraspecific variabilities. The results indicated that IGS1 sequences and PCR-RFLPs can be used for species identification of T. inkin, while RAPD of genomic DNA is more suitable for intraspecific genotyping. |
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| Keywords: | rRNA gene sequencing PCR-restriction fragment length polymorphism random amplified polymorphism |
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