两种光照处理下苦荞芽菜的代谢物分析

以黑暗和光照处理下苦荞（Fagopyrum tataricum（L.）Gaertner）品种‘晋荞2号’种子萌发所得的芽菜为材料，利用气相色谱-质谱联用分析技术，对两种处理下苦荞芽菜代谢产物的差异进行分析。结果显示，两种光照处理下苦荞芽菜的代谢产物差异明显，在芽菜中共检测出383种代谢产物，其中不同光照处理造成了137种代谢产物的显著差异，他们主要集中在氨基酸及其衍生物、糖类及其衍生物、芳香族化合物和脂肪酸等。除氨基酸及其衍生物外，其余绝大多数差异代谢物的含量在光照组芽菜中较高；KEGG代谢通路分析结果表明，差异代谢物富集于136条物质代谢通路。说明光照能够促进苦荞芽菜多数营养代谢物的生成和积累，营养组分较暗培养更为丰富，营养价值更高。

Metabolite analysis of Fagopyrum tataricum (L.) Gaertner sprouts treated by two kinds of illumination

Fagopyrum tataricum (L.) Gaertner (variety ‘Jinqiao 2’) sprouts obtained from seed germination under dark and light treatment were analyzed by gas chromatography-mass spectrometry to determine differences in metabolite content. Results showed that tartary buckwheat sprout metabolites were significantly different under the two light treatments. A total of 383 metabolites were detected, among which 137 metabolites were significantly different under the different light treatments, particularly amino acids and their derivatives, carbohydrates and their derivatives, aromatic compounds, and fatty acids. Except for amino acids and their derivatives, all other differential metabolites were found at a higher concentration in the light-irradiated sprouts. KEGG metabolic pathway analysis showed that the different metabolites were enriched in 136 metabolic pathways. Thus, our study showed that light promoted the production and accumulation of most nutrient metabolites in F. tataricum sprouts, and the nutrient components were richer and more valuable than those under dark culture.